Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient <it>in vitro </it>discrimination and restriction of a single nucleotide polymorphism
<p>Abstract</p> <p>Background</p> <p>Many SNP discrimination strategies employ natural restriction endonucleases to discriminate between allelic states. However, SNPs are often not associated with a restriction site and therefore, a number of attempts have been made to...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-08-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/11/83 |