Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles
Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of C...
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doaj-e414b63557574a759f1d8d10187e2a952020-12-08T08:40:27ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-11-01810.3389/fcell.2020.592518592518Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular VesiclesJoana Maia0Joana Maia1Andreia Hanada Otake2Andreia Hanada Otake3Juliana Poças4Juliana Poças5Juliana Poças6Ana Sofia Carvalho7Hans Christian Beck8Ana Magalhães9Ana Magalhães10Rune Matthiesen11Maria Carolina Strano Moraes12Bruno Costa-Silva13Champalimaud Centre for the Unknown, Champalimaud Foundation, Lisbon, PortugalGraduate Program in Areas of Basic and Applied Biology, University of Porto, Porto, PortugalChampalimaud Centre for the Unknown, Champalimaud Foundation, Lisbon, PortugalCenter for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazili3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, PortugalIPATIMUP – Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, PortugalInstituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, PortugalComputational and Experimental Biology Group, CEDOC, Chronic Diseases Research Centre, NOVA Medical School, Faculdade de Ciencias Medicas, Universidade NOVA de Lisboa, Lisbon, PortugalCentre for Clinical Proteomics, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmarki3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, PortugalIPATIMUP – Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, PortugalComputational and Experimental Biology Group, CEDOC, Chronic Diseases Research Centre, NOVA Medical School, Faculdade de Ciencias Medicas, Universidade NOVA de Lisboa, Lisbon, PortugalChampalimaud Centre for the Unknown, Champalimaud Foundation, Lisbon, PortugalChampalimaud Centre for the Unknown, Champalimaud Foundation, Lisbon, PortugalPancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM cells and induce the expansion of this cell population. Transcriptomic characterization of these cells shows that PC-EVs upregulate IgG and IgA genes, which have been linked to the presence of monocytes/macrophages in tumor microenvironments. We also report here the transcriptional downregulation of genes linked to monocyte/macrophage activation, trafficking, and expression of inflammatory molecules. Together, these results show for the first time the existence of a PC–BM communication axis mediated by EVs with a potential role in PC tumor microenvironments.https://www.frontiersin.org/articles/10.3389/fcell.2020.592518/fulltumor microenvironmentextracellular vesiclesmacrophagesmonocytesmetastasispancreatic cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joana Maia Joana Maia Andreia Hanada Otake Andreia Hanada Otake Juliana Poças Juliana Poças Juliana Poças Ana Sofia Carvalho Hans Christian Beck Ana Magalhães Ana Magalhães Rune Matthiesen Maria Carolina Strano Moraes Bruno Costa-Silva |
spellingShingle |
Joana Maia Joana Maia Andreia Hanada Otake Andreia Hanada Otake Juliana Poças Juliana Poças Juliana Poças Ana Sofia Carvalho Hans Christian Beck Ana Magalhães Ana Magalhães Rune Matthiesen Maria Carolina Strano Moraes Bruno Costa-Silva Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles Frontiers in Cell and Developmental Biology tumor microenvironment extracellular vesicles macrophages monocytes metastasis pancreatic cancer |
author_facet |
Joana Maia Joana Maia Andreia Hanada Otake Andreia Hanada Otake Juliana Poças Juliana Poças Juliana Poças Ana Sofia Carvalho Hans Christian Beck Ana Magalhães Ana Magalhães Rune Matthiesen Maria Carolina Strano Moraes Bruno Costa-Silva |
author_sort |
Joana Maia |
title |
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles |
title_short |
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles |
title_full |
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles |
title_fullStr |
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles |
title_full_unstemmed |
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles |
title_sort |
transcriptome reprogramming of cd11b+ bone marrow cells by pancreatic cancer extracellular vesicles |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-11-01 |
description |
Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM cells and induce the expansion of this cell population. Transcriptomic characterization of these cells shows that PC-EVs upregulate IgG and IgA genes, which have been linked to the presence of monocytes/macrophages in tumor microenvironments. We also report here the transcriptional downregulation of genes linked to monocyte/macrophage activation, trafficking, and expression of inflammatory molecules. Together, these results show for the first time the existence of a PC–BM communication axis mediated by EVs with a potential role in PC tumor microenvironments. |
topic |
tumor microenvironment extracellular vesicles macrophages monocytes metastasis pancreatic cancer |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2020.592518/full |
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