Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation.
DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a...
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Online Access: | https://doi.org/10.1371/journal.pone.0232724 |
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doaj-e43f193ac583497686a8c7c8372e6cbd2021-03-03T21:47:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023272410.1371/journal.pone.0232724Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation.Hirofumi KuritaNatsuki HarutaYoshito UchihashiTakahito SetoKazunori TakashimaDNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.https://doi.org/10.1371/journal.pone.0232724 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hirofumi Kurita Natsuki Haruta Yoshito Uchihashi Takahito Seto Kazunori Takashima |
spellingShingle |
Hirofumi Kurita Natsuki Haruta Yoshito Uchihashi Takahito Seto Kazunori Takashima Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. PLoS ONE |
author_facet |
Hirofumi Kurita Natsuki Haruta Yoshito Uchihashi Takahito Seto Kazunori Takashima |
author_sort |
Hirofumi Kurita |
title |
Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. |
title_short |
Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. |
title_full |
Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. |
title_fullStr |
Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. |
title_full_unstemmed |
Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. |
title_sort |
strand breaks and chemical modification of intracellular dna induced by cold atmospheric pressure plasma irradiation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation. |
url |
https://doi.org/10.1371/journal.pone.0232724 |
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