Analytical and Clinical Validation for RT-qPCR Detection of SARS-CoV-2 Without RNA Extraction

Background: The recent COVID-19 pandemic has posed an unprecedented challenge to laboratory diagnosis, based on the amplification of SARS-CoV-2 RNA. With global contagion figures exceeding 4 million persons, the shortage of reagents for RNA extraction represents a bottleneck for testing globally. We...

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Bibliographic Details
Main Authors: José P. Miranda, Javiera Osorio, Mauricio Videla, Gladys Angel, Rossana Camponovo, Marcela Henríquez-Henríquez
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Medicine
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmed.2020.567572/full