The Central Role of IFI204 in IFN-β Release and Autophagy Activation during Mycobacterium bovis Infection

• Main Authors: Liu Chunfa, Sun Xin, Li Qiang, Srinand Sreevatsan, Lifeng Yang, Deming Zhao, Xiangmei Zhou
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2017-05-01
• Series: Frontiers in Cellular and Infection Microbiology
• Subjects: IFI204; IFN-β; M. bovis; autophagy; DNA sensor
• Online Access: http://journal.frontiersin.org/article/10.3389/fcimb.2017.00169/full

Mycobacterium bovis (M. bovis) is the pathogen of animals and humans that can replicate in the phagosomes of myeloid cells. Cytosolic detection of bacterial products plays a crucial role in initiating the innate immune response, including autophagy activation and interferon-β (IFN-β) release. Although IFN-β release and autophagy activation have been reported during mycobacterium infection, the mechanisms underlying remains poorly defined. Here, we demonstrated that IFN-β release increases in macrophages exposed to M. bovis and this requires the activation of the DNA sensor of interferon-γ inducible protein 204 (IFI204). Knockdown of the IFI204 in immortalized and primary murine macrophages blocked IFN-β production and autophagy marker LC3 expression. Thus, our results indicate that the IFI204 is an important sensor for innate immune responses of M. bovis infection.