A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts

A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts

Summary: This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to p...

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Bibliographic Details
Main Authors: Gabriella E. Farrugia, Micheal A. McLellan, Kate L. Weeks, Aya Matsumoto, Charles D. Cohen, Crisdion Krstevski, Taylah L. Gaynor, Adam C. Parslow, Julie R. McMullen, Alexander R. Pinto
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Cell separation/fractionation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005724

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http://www.sciencedirect.com/science/article/pii/S2666166721005724

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