Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients

Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients

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Since SARS-CoV-2-based disease (COVID-19) spreads as a pandemic, the necessity of a highly sensitive molecular diagnosis that can drastically reduce false negatives reverse transcription PCR (rtPCR) results, raises as a major clinical need. Here we evaluated the performance of a ddPCR-based assay to...

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Bibliographic Details
Main Authors: Claudia Alteri, Valeria Cento, Maria Antonello, Luna Colagrossi, Marco Merli, Nicola Ughi, Silvia Renica, Elisa Matarazzo, Federica Di Ruscio, Livia Tartaglione, Jacopo Colombo, Chiara Grimaldi, Stefania Carta, Alice Nava, Valentino Costabile, Chiara Baiguera, Daniela Campisi, Diana Fanti, Chiara Vismara, Roberto Fumagalli, Francesco Scaglione, Oscar Massimiliano Epis, Massimo Puoti, Carlo Federico Perno, Timothy D. Minogue
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478621/?tool=EBI

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478621/?tool=EBI

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