Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simpli...

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Bibliographic Details
Main Authors: Andrea von Groll, Anandi Martin, Françoise Portaels, Pedro Eduardo Almeida da Silva, Juan Carlos Palomino
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2010-06-01
Series:Brazilian Journal of Microbiology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200006