Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Abstract Background Sample index cross-talk can result in false positive calls when massively parallel sequencing (MPS) is used for sensitive applications such as low-frequency somatic variant discovery, ancient DNA investigations, microbial detection in human samples, or circulating cell-free tumor...

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Bibliographic Details
Main Authors: Laura E. MacConaill, Robert T. Burns, Anwesha Nag, Haley A. Coleman, Michael K. Slevin, Kristina Giorda, Madelyn Light, Kevin Lai, Mirna Jarosz, Matthew S. McNeill, Matthew D. Ducar, Matthew Meyerson, Aaron R. Thorner
Format: Article
Language:English
Published: BMC 2018-01-01
Series:BMC Genomics
Subjects:
Next generation sequencing
Massively parallel sequencing
Adapter
Index
Barcode cross-talk
Illumina
Online Access:http://link.springer.com/article/10.1186/s12864-017-4428-5

Internet

http://link.springer.com/article/10.1186/s12864-017-4428-5

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