Mixed pyruvate labeling enables backbone resonance assignment of large proteins using a single experiment
Structure determination of large proteins by solution state NMR is challenging due to spectral overlap. Here the authors present a labeling strategy using 2-13C and 3-13C pyruvate as carbon source for E. coli, which increases the effective resolution of triple-resonance HNCA experiments and helps to...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-02767-8 |