The effect of MEP pathway and other inhibitors on the intracellular localization of a plasma membrane-targeted, isoprenylable GFP reporter protein in tobacco BY-2 cells [v2; ref status: indexed, http://f1000r.es/2af]

We have established an in vivo visualization system for the geranylgeranylation of proteins in a stably transformed tobacco BY-2 cell line, based on the expression of a dexamethasone-inducible GFP fused to the carboxy-terminal basic domain of the rice calmodulin CaM61, which naturally bears a CaaL g...

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Bibliographic Details
Main Authors: Michael Hartmann, Andrea Hemmerlin, Elisabet Gas-Pascual, Esther Gerber, Denis Tritsch, Michel Rohmer, Thomas J Bach
Format: Article
Language:English
Published: F1000 Research Ltd 2013-11-01
Series:F1000Research
Subjects:
Online Access:http://f1000research.com/articles/2-170/v2