Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models
Rabies, a fatal disease in humans and other mammals, is caused by the rabies virus (RABV), and it poses a public health threat in many parts of the world. Once symptoms of rabies appear, the mortality is near 100%. There is currently no effective treatment for rabies. In our study, two human-derived...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.702273/full |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Fei Huang Fei Huang Meishen Ren Meishen Ren Jie Pei Jie Pei Hong Mei Hong Mei Baokun Sui Baokun Sui Qiong Wu Qiong Wu Benjie Chai Benjie Chai Ruicheng Yang Ruicheng Yang Ming Zhou Ming Zhou Zhen F. Fu Zhen F. Fu Huiping Zhou Ling Zhao Ling Zhao |
| spellingShingle |
Fei Huang Fei Huang Meishen Ren Meishen Ren Jie Pei Jie Pei Hong Mei Hong Mei Baokun Sui Baokun Sui Qiong Wu Qiong Wu Benjie Chai Benjie Chai Ruicheng Yang Ruicheng Yang Ming Zhou Ming Zhou Zhen F. Fu Zhen F. Fu Huiping Zhou Ling Zhao Ling Zhao Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models Frontiers in Microbiology rabies viruses adeno-associated virus virus-neutralizing antibody preexposure prophylaxis postexposure prophylaxis rodent models |
| author_facet |
Fei Huang Fei Huang Meishen Ren Meishen Ren Jie Pei Jie Pei Hong Mei Hong Mei Baokun Sui Baokun Sui Qiong Wu Qiong Wu Benjie Chai Benjie Chai Ruicheng Yang Ruicheng Yang Ming Zhou Ming Zhou Zhen F. Fu Zhen F. Fu Huiping Zhou Ling Zhao Ling Zhao |
| author_sort |
Fei Huang |
| title |
Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models |
| title_short |
Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models |
| title_full |
Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models |
| title_fullStr |
Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models |
| title_full_unstemmed |
Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models |
| title_sort |
preexposure and postexposure prophylaxis of rabies with adeno-associated virus expressing virus-neutralizing antibody in rodent models |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Microbiology |
| issn |
1664-302X |
| publishDate |
2021-08-01 |
| description |
Rabies, a fatal disease in humans and other mammals, is caused by the rabies virus (RABV), and it poses a public health threat in many parts of the world. Once symptoms of rabies appear, the mortality is near 100%. There is currently no effective treatment for rabies. In our study, two human-derived RABV-neutralizing antibodies (RVNA), CR57 and CR4098, were cloned into adeno-associated virus (AAV) vectors, and recombinant AAVs expressing RVNA were evaluated for postexposure prophylaxis after intrathecal injection into RABV-infected rats. At 4days post-infection with a lethal dose of RABV, 60% of the rats that received an intrathecal injection of AAV-CR57 survived, while 100% of the rats inoculated with AAV-enhanced green fluorescent protein (EGFP) succumbed to rabies. Overall, these results demonstrate that AAV-encoding RVNA can be utilized as a potential human rabies postexposure prophylaxis. |
| topic |
rabies viruses adeno-associated virus virus-neutralizing antibody preexposure prophylaxis postexposure prophylaxis rodent models |
| url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.702273/full |
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doaj-e606c9705380433fb38f6bfb6d4e55ed2021-08-19T15:07:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.702273702273Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent ModelsFei Huang0Fei Huang1Meishen Ren2Meishen Ren3Jie Pei4Jie Pei5Hong Mei6Hong Mei7Baokun Sui8Baokun Sui9Qiong Wu10Qiong Wu11Benjie Chai12Benjie Chai13Ruicheng Yang14Ruicheng Yang15Ming Zhou16Ming Zhou17Zhen F. Fu18Zhen F. Fu19Huiping Zhou20Ling Zhao21Ling Zhao22State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaSchool of Basic Medicine, Hubei University of Science and Technology, Xianning, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaRabies, a fatal disease in humans and other mammals, is caused by the rabies virus (RABV), and it poses a public health threat in many parts of the world. Once symptoms of rabies appear, the mortality is near 100%. There is currently no effective treatment for rabies. In our study, two human-derived RABV-neutralizing antibodies (RVNA), CR57 and CR4098, were cloned into adeno-associated virus (AAV) vectors, and recombinant AAVs expressing RVNA were evaluated for postexposure prophylaxis after intrathecal injection into RABV-infected rats. At 4days post-infection with a lethal dose of RABV, 60% of the rats that received an intrathecal injection of AAV-CR57 survived, while 100% of the rats inoculated with AAV-enhanced green fluorescent protein (EGFP) succumbed to rabies. Overall, these results demonstrate that AAV-encoding RVNA can be utilized as a potential human rabies postexposure prophylaxis.https://www.frontiersin.org/articles/10.3389/fmicb.2021.702273/fullrabies virusesadeno-associated virusvirus-neutralizing antibodypreexposure prophylaxispostexposure prophylaxisrodent models |