MYC Immunohistochemistry Predicts MYC Rearrangements by FISH
MYC is the proto-oncogene classically associated with Burkitt lymphoma (BL) located at chromosomal locus 8q24. Rearrangements of MYC are seen in nearly 100% of BL but have been reported in 3–16% of diffuse large B-cell lymphomas (DLBCLs). Rearrangements of MYC are tested for by flourescence in situ...
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doaj-e61ec7afa24b4ce1adf9917c96bf75f02020-11-25T02:29:36ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2017-09-01710.3389/fonc.2017.00209289439MYC Immunohistochemistry Predicts MYC Rearrangements by FISHJulum Nwanze0Julum Nwanze1Momin T. Siddiqui2Keith A. Stevens3Debra Saxe4Cynthia Cohen5Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, United StatesDepartment of Pathology and Laboratory Medicine, Tulane University Hospital, New Orleans, LA, United StatesDepartment of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, United StatesDepartment of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, United StatesDepartment of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, United StatesDepartment of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, United StatesMYC is the proto-oncogene classically associated with Burkitt lymphoma (BL) located at chromosomal locus 8q24. Rearrangements of MYC are seen in nearly 100% of BL but have been reported in 3–16% of diffuse large B-cell lymphomas (DLBCLs). Rearrangements of MYC are tested for by flourescence in situ hybridization (FISH). In this study, we compared immunohistochemistry (IHC) using a monoclonal antibody directed against the human Myc protein to the current method, FISH. 31 cases were identified that had been tested for MYC rearrangements by FISH over 27 months with heterogeneity in the diagnoses: 5 BL; 10 DLBCL; 3 B-cell lymphoma unclassifiable between DLBCL and BL; 5 B-cell lymphoma not otherwise specified; 1 EBV-related B-cell lymphoma; 1 composite CLL/SLL-large cell lymphoma; and 6 designated as high-grade or aggressive B-cell lymphoma. Analysis by FISH was performed as part of the clinical workup, where a MYC rearrangement is defined as a split fusion signal in at least 5.7% of cells. Myc-IHC was interpreted as a qualitative positive (overexpressed) or negative (not overexpressed) result. 12 cases (39%) were positive for MYC rearrangements by FISH. Overall, 13 cases (42%) showed Myc overexpression by IHC, 11 of which harbored a MYC rearrangement by FISH. There were two false positives and one false negative. Thus, Myc-IHC predicted a MYC rearrangement by FISH with 92% sensitivity and 89% specificity. We can thus conclude that Myc-IHC should be a potentially useful screening tool for identifying lymphomas that may harbor a MYC rearrangement.http://journal.frontiersin.org/article/10.3389/fonc.2017.00209/fullMYCimmunohistochemistryBurkitt lymphomain situ hybridizationMYC rearrangement |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julum Nwanze Julum Nwanze Momin T. Siddiqui Keith A. Stevens Debra Saxe Cynthia Cohen |
spellingShingle |
Julum Nwanze Julum Nwanze Momin T. Siddiqui Keith A. Stevens Debra Saxe Cynthia Cohen MYC Immunohistochemistry Predicts MYC Rearrangements by FISH Frontiers in Oncology MYC immunohistochemistry Burkitt lymphoma in situ hybridization MYC rearrangement |
author_facet |
Julum Nwanze Julum Nwanze Momin T. Siddiqui Keith A. Stevens Debra Saxe Cynthia Cohen |
author_sort |
Julum Nwanze |
title |
MYC Immunohistochemistry Predicts MYC Rearrangements by FISH |
title_short |
MYC Immunohistochemistry Predicts MYC Rearrangements by FISH |
title_full |
MYC Immunohistochemistry Predicts MYC Rearrangements by FISH |
title_fullStr |
MYC Immunohistochemistry Predicts MYC Rearrangements by FISH |
title_full_unstemmed |
MYC Immunohistochemistry Predicts MYC Rearrangements by FISH |
title_sort |
myc immunohistochemistry predicts myc rearrangements by fish |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2017-09-01 |
description |
MYC is the proto-oncogene classically associated with Burkitt lymphoma (BL) located at chromosomal locus 8q24. Rearrangements of MYC are seen in nearly 100% of BL but have been reported in 3–16% of diffuse large B-cell lymphomas (DLBCLs). Rearrangements of MYC are tested for by flourescence in situ hybridization (FISH). In this study, we compared immunohistochemistry (IHC) using a monoclonal antibody directed against the human Myc protein to the current method, FISH. 31 cases were identified that had been tested for MYC rearrangements by FISH over 27 months with heterogeneity in the diagnoses: 5 BL; 10 DLBCL; 3 B-cell lymphoma unclassifiable between DLBCL and BL; 5 B-cell lymphoma not otherwise specified; 1 EBV-related B-cell lymphoma; 1 composite CLL/SLL-large cell lymphoma; and 6 designated as high-grade or aggressive B-cell lymphoma. Analysis by FISH was performed as part of the clinical workup, where a MYC rearrangement is defined as a split fusion signal in at least 5.7% of cells. Myc-IHC was interpreted as a qualitative positive (overexpressed) or negative (not overexpressed) result. 12 cases (39%) were positive for MYC rearrangements by FISH. Overall, 13 cases (42%) showed Myc overexpression by IHC, 11 of which harbored a MYC rearrangement by FISH. There were two false positives and one false negative. Thus, Myc-IHC predicted a MYC rearrangement by FISH with 92% sensitivity and 89% specificity. We can thus conclude that Myc-IHC should be a potentially useful screening tool for identifying lymphomas that may harbor a MYC rearrangement. |
topic |
MYC immunohistochemistry Burkitt lymphoma in situ hybridization MYC rearrangement |
url |
http://journal.frontiersin.org/article/10.3389/fonc.2017.00209/full |
work_keys_str_mv |
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