Replicates, read numbers and other important experimental design considerations for microbial RNA-seq identified using Bacillus thuringiensis datasets

Replicates, read numbers and other important experimental design considerations for microbial RNA-seq identified using Bacillus thuringiensis datasets

RNA-seq is being used increasingly for gene expression studies and it is revolutionizing the fields of genomics and transcriptomics. However, the field of RNA-seq analysis is still evolving. Therefore, we specifically designed this study to contain large numbers of reads and four biological replicat...

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Bibliographic Details
Main Authors: Punita eManga, Dawn M Klingeman, Tse-Yuan S Lu, Tonia L Mehlhorn, Dale A Pelletier, Loren J Hauser, Charlotte M Wilson, Steven D. Brown
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-05-01
Series:Frontiers in Microbiology
Subjects:
coverage
normalization
Illumina
Negative Binomial
Replicates
DESeq2
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00794/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00794/full

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