2PE-STED microscopy with a single Ti:sapphire laser for reduced illumination.

We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input p...

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Bibliographic Details
Main Authors: Qifeng Li, Yang Wang, Da Chen, Sherry S H Wu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3916425?pdf=render