Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus

It is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.

Bibliographic Details
Main Authors:	Xi Wang, Xintian You, Julian D. Langer, Jingyi Hou, Fiona Rupprecht, Irena Vlatkovic, Claudia Quedenau, Georgi Tushev, Irina Epstein, Bernhard Schaefke, Wei Sun, Liang Fang, Guipeng Li, Yuhui Hu, Erin M. Schuman, Wei Chen
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-13037-0

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spelling	doaj-e648ad9d85e9487fb44af5a9caede55c2021-05-11T11:49:14ZengNature Publishing GroupNature Communications2041-17232019-11-0110111510.1038/s41467-019-13037-0Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampusXi Wang0Xintian You1Julian D. Langer2Jingyi Hou3Fiona Rupprecht4Irena Vlatkovic5Claudia Quedenau6Georgi Tushev7Irina Epstein8Bernhard Schaefke9Wei Sun10Liang Fang11Guipeng Li12Yuhui Hu13Erin M. Schuman14Wei Chen15Max Delbrück Center for Molecular MedicineMax Delbrück Center for Molecular MedicineMax Planck Institute for Brain ResearchMax Delbrück Center for Molecular MedicineMax Planck Institute for Brain ResearchMax Planck Institute for Brain ResearchMax Delbrück Center for Molecular MedicineMax Planck Institute for Brain ResearchMax Planck Institute for Brain ResearchDepartment of Biology, Southern University of Science and TechnologyDepartment of Biology, Southern University of Science and TechnologyDepartment of Biology, Southern University of Science and TechnologyDepartment of Biology, Southern University of Science and TechnologyDepartment of Biology, Southern University of Science and TechnologyMax Planck Institute for Brain ResearchDepartment of Biology, Southern University of Science and TechnologyIt is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.https://doi.org/10.1038/s41467-019-13037-0
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xi Wang Xintian You Julian D. Langer Jingyi Hou Fiona Rupprecht Irena Vlatkovic Claudia Quedenau Georgi Tushev Irina Epstein Bernhard Schaefke Wei Sun Liang Fang Guipeng Li Yuhui Hu Erin M. Schuman Wei Chen
spellingShingle	Xi Wang Xintian You Julian D. Langer Jingyi Hou Fiona Rupprecht Irena Vlatkovic Claudia Quedenau Georgi Tushev Irina Epstein Bernhard Schaefke Wei Sun Liang Fang Guipeng Li Yuhui Hu Erin M. Schuman Wei Chen Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus Nature Communications
author_facet	Xi Wang Xintian You Julian D. Langer Jingyi Hou Fiona Rupprecht Irena Vlatkovic Claudia Quedenau Georgi Tushev Irina Epstein Bernhard Schaefke Wei Sun Liang Fang Guipeng Li Yuhui Hu Erin M. Schuman Wei Chen
author_sort	Xi Wang
title	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_short	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_full	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_fullStr	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_full_unstemmed	Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus
title_sort	full-length transcriptome reconstruction reveals a large diversity of rna and protein isoforms in rat hippocampus
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-11-01
description	It is challenging to characterize diverse transcript isoforms by short-read sequencing. Here the authors report full-length transcriptomes in rat hippocampus by hybrid-sequencing, predict isoform-specific translational status, and reconstruct open reading frames validated by mass spectrometry.
url	https://doi.org/10.1038/s41467-019-13037-0
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Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus

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