Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults

Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults

Abstract Background Volunteer infection studies have become a standard model for evaluating drug efficacy against Plasmodium infections. Molecular techniques such as qPCR are used in these studies due to their ability to provide robust and accurate estimates of parasitaemia at increased sensitivity...

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Bibliographic Details
Main Authors: Claire Y. T. Wang, Emma L. Ballard, Zuleima Pava, Louise Marquart, Jane Gaydon, Sean C. Murphy, David Whiley, Peter O’Rourke, James S. McCarthy
Format: Article
Language:English
Published: BMC 2021-04-01
Series:Malaria Journal
Subjects:
Plasmodium falciparum
Hydrolysis probe
Quantitative PCR
Validation
Volunteer infection studies
MIQE
Online Access:https://doi.org/10.1186/s12936-021-03717-y

Internet

https://doi.org/10.1186/s12936-021-03717-y

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