Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.

Bibliographic Details
Main Authors: Danai Laskaratou, Guillermo Solís Fernández, Quinten Coucke, Eduard Fron, Susana Rocha, Johan Hofkens, Jelle Hendrix, Hideaki Mizuno
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-22816-7

Internet

https://doi.org/10.1038/s41467-021-22816-7

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