Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.

Bibliographic Details
Main Authors:	Danai Laskaratou, Guillermo Solís Fernández, Quinten Coucke, Eduard Fron, Susana Rocha, Johan Hofkens, Jelle Hendrix, Hideaki Mizuno
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-05-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-22816-7

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spelling	doaj-e69808e0f8e94533a612962a43ef72cd2021-05-09T11:12:44ZengNature Publishing GroupNature Communications2041-17232021-05-0112111210.1038/s41467-021-22816-7Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donorDanai Laskaratou0Guillermo Solís Fernández1Quinten Coucke2Eduard Fron3Susana Rocha4Johan Hofkens5Jelle Hendrix6Hideaki Mizuno7Laboratory for Biomolecular Network Dynamics, Biochemistry, Molecular and Structural Biology Section, Department of ChemistryChem & Tech-Molecular Imaging and Photonics, Department of ChemistryChem & Tech-Molecular Imaging and Photonics, Department of ChemistryChem & Tech-Molecular Imaging and Photonics, Department of ChemistryChem & Tech-Molecular Imaging and Photonics, Department of ChemistryChem & Tech-Molecular Imaging and Photonics, Department of ChemistryChem & Tech-Molecular Imaging and Photonics, Department of ChemistryLaboratory for Biomolecular Network Dynamics, Biochemistry, Molecular and Structural Biology Section, Department of ChemistryThe FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.https://doi.org/10.1038/s41467-021-22816-7
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Danai Laskaratou Guillermo Solís Fernández Quinten Coucke Eduard Fron Susana Rocha Johan Hofkens Jelle Hendrix Hideaki Mizuno
spellingShingle	Danai Laskaratou Guillermo Solís Fernández Quinten Coucke Eduard Fron Susana Rocha Johan Hofkens Jelle Hendrix Hideaki Mizuno Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor Nature Communications
author_facet	Danai Laskaratou Guillermo Solís Fernández Quinten Coucke Eduard Fron Susana Rocha Johan Hofkens Jelle Hendrix Hideaki Mizuno
author_sort	Danai Laskaratou
title	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_short	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_full	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_fullStr	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_full_unstemmed	Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
title_sort	quantification of fret-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-05-01
description	The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.
url	https://doi.org/10.1038/s41467-021-22816-7
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Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

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