Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
The FRET efficiency usually predominantly depends on the proximity of donor and acceptor. Here the authors report an anisotropy-based mode of FRET detection, FRET-induced Angular Displacement Evaluation via Dim donor (FADED), to allow quantification of the relative angle between donor and acceptor.
Main Authors: | Danai Laskaratou, Guillermo Solís Fernández, Quinten Coucke, Eduard Fron, Susana Rocha, Johan Hofkens, Jelle Hendrix, Hideaki Mizuno |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-05-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-021-22816-7 |
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