An improved protocol for intact chloroplasts and cpDNA isolation in conifers.

An improved protocol for intact chloroplasts and cpDNA isolation in conifers.

BACKGROUND:Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing prot...

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Bibliographic Details
Main Authors: Leila do Nascimento Vieira, Helisson Faoro, Hugo Pacheco de Freitas Fraga, Marcelo Rogalski, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Rubens Onofre Nodari, Miguel Pedro Guerra
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24392157/?tool=EBI

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24392157/?tool=EBI

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