A low temperature flotation method to rapidly isolate lipoproteins from plasma

To minimize oxidative modification, a low temperature, sequential flotation method was developed to isolate plasma lipoproteins in 18 h using a benchtop ultracentrifuge. The protein distributions were characterized using agarose and SDS-polyacrylamide gel electrophoresis, and an SDS-Lowry protein as...

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Bibliographic Details
Main Authors: H. Tong, H.R. Knapp, M. VanRollins
Format: Article
Language:English
Published: Elsevier 1998-08-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520322008