A low temperature flotation method to rapidly isolate lipoproteins from plasma
To minimize oxidative modification, a low temperature, sequential flotation method was developed to isolate plasma lipoproteins in 18 h using a benchtop ultracentrifuge. The protein distributions were characterized using agarose and SDS-polyacrylamide gel electrophoresis, and an SDS-Lowry protein as...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1998-08-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520322008 |