Enzymatic determination of bile acids from liver cells with 3 alpha-hydroxysteroid dehydrogenase--a warning.
3 alpha-Hydroxysteroid dehydrogenase was used to monitor bile acid production by rat hepatocyte suspensions. Several observations suggested that the method lacked specificity and detected some other metabolites besides bile acids. This was due to a malate dehydrogenase activity present in four diffe...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1981-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520373417 |