Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells
The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1β (IL-1 β)....
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doaj-e739c05a493e4bae8a016b71960b856e2021-04-30T07:16:45ZengElsevierJournal of Functional Foods1756-46462019-08-0159329336Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cellsAlberto Burgos-Edwards0Laura Martín-Pérez1Felipe Jiménez-Aspee2Cristina Theoduloz3Guillermo Schmeda-Hirschmann4Mar Larrosa5Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, 3460000 Talca, ChileUniversidad Europea de Madrid, Villaviciosa de Odón, Madrid 28670, Spain; Instituto de Investigación Hospital, 12 de Octubre (i + 12), Madrid, SpainDepartamento de Ciencias Básicas Biomédicas, Facultad de Ciencias de la Salud, Universidad de Talca, 3460000 Talca, ChileLaboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca, 3460000 Talca, ChileLaboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, 3460000 Talca, ChileNutrition, Microbiota and Health Group, Faculty of Biomedical Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, Madrid 28670, Spain; Corresponding author at: Nutrition, Microbiota and Health Group, Faculty of Biomedical Sciences, Universidad Europea de Madrid, Spain.The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1β (IL-1 β). The inhibitory effect of non-digested and digested PEEs towards human cyclooxygenase 1 (COX-1) and COX-2, and the gene expression of COX-2 and inducible nitric oxide synthase (iNOS) was also evaluated. The digested PEE from R. punctatum decreased the secretion of IL-8, IL-6, and TNF-α; whereas R. magellanicum reduced IL-6 and TNF-α in the Caco-2 cells (p < 0.05). Both digested extracts significantly down-regulated the mRNA expression of COX-2 and iNOS (p < 0.05). PEEs showed 60% of inhibition towards COXs, with higher inhibition against COX-2. The PEEs from R. punctatum displayed better anti-inflammatory activity in all the experiments. Our results suggest that R. magellanicum and R. punctatum might be useful against intestine inflammatory conditions.http://www.sciencedirect.com/science/article/pii/S1756464619303226Ribes spp.Intestinal inflammationCOX-2iNOSInterleukin secretion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alberto Burgos-Edwards Laura Martín-Pérez Felipe Jiménez-Aspee Cristina Theoduloz Guillermo Schmeda-Hirschmann Mar Larrosa |
spellingShingle |
Alberto Burgos-Edwards Laura Martín-Pérez Felipe Jiménez-Aspee Cristina Theoduloz Guillermo Schmeda-Hirschmann Mar Larrosa Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells Journal of Functional Foods Ribes spp. Intestinal inflammation COX-2 iNOS Interleukin secretion |
author_facet |
Alberto Burgos-Edwards Laura Martín-Pérez Felipe Jiménez-Aspee Cristina Theoduloz Guillermo Schmeda-Hirschmann Mar Larrosa |
author_sort |
Alberto Burgos-Edwards |
title |
Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells |
title_short |
Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells |
title_full |
Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells |
title_fullStr |
Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells |
title_full_unstemmed |
Anti-inflammatory effect of polyphenols from Chilean currants (Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells |
title_sort |
anti-inflammatory effect of polyphenols from chilean currants (ribes magellanicum and r. punctatum) after in vitro gastrointestinal digestion on caco-2 cells |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2019-08-01 |
description |
The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1β (IL-1 β). The inhibitory effect of non-digested and digested PEEs towards human cyclooxygenase 1 (COX-1) and COX-2, and the gene expression of COX-2 and inducible nitric oxide synthase (iNOS) was also evaluated. The digested PEE from R. punctatum decreased the secretion of IL-8, IL-6, and TNF-α; whereas R. magellanicum reduced IL-6 and TNF-α in the Caco-2 cells (p < 0.05). Both digested extracts significantly down-regulated the mRNA expression of COX-2 and iNOS (p < 0.05). PEEs showed 60% of inhibition towards COXs, with higher inhibition against COX-2. The PEEs from R. punctatum displayed better anti-inflammatory activity in all the experiments. Our results suggest that R. magellanicum and R. punctatum might be useful against intestine inflammatory conditions. |
topic |
Ribes spp. Intestinal inflammation COX-2 iNOS Interleukin secretion |
url |
http://www.sciencedirect.com/science/article/pii/S1756464619303226 |
work_keys_str_mv |
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