Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

Accumulation of proline is a widespread plant response to a broad range of environmental stress conditions including salt and osmotic stress. Proline accumulation is achieved mainly by upregulation of proline biosynthesis in the cytosol and by inhibition of proline degradation in mitochondria. Chang...

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Bibliographic Details
Main Authors: Sandrine Lebreton, Cécile Cabassa-Hourton, Arnould Savouré, Dietmar Funck, Giuseppe Forlani
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Plant Science
Subjects:
proline dehydrogenase
pyrroline-5-carboxylate reductase
enzyme activity assay
electron acceptor
protein extraction
mitochondria
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2020.602939/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2020.602939/full

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