Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method

Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min. HAC facilitates insert integration due to a sequence alignment strategy, by way of short...

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Bibliographic Details
Main Authors: Lendl Tan, Emily J. Strong, Kyra Woods, Nicholas P. West
Format: Article
Language:English
Published: PeerJ Inc. 2018-06-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/5146.pdf