Rapid and selective detection of fatty acylated proteins using ω-alkynyl-fatty acids and click chemistry[S]

Rapid and selective detection of fatty acylated proteins using ω-alkynyl-fatty acids and click chemistry[S]

Progress in understanding the biology of protein fatty acylation has been impeded by the lack of rapid direct detection and identification methods. We first report that a synthetic ω-alkynyl-palmitate analog can be readily and specifically incorporated into GAPDH or mitochondrial 3-hydroxyl-3-methyl...

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Bibliographic Details
Main Authors: Megan C. Yap, Morris A. Kostiuk, Dale D.O. Martin, Maneka A. Perinpanayagam, Pieter G. Hak, Anjaiah Siddam, Janaki R. Majjigapu, Gurram Rajaiah, Bernd O. Keller, Jennifer A. Prescher, Peng Wu, Carolyn R. Bertozzi, John R. Falck, Luc G. Berthiaume
Format: Article
Language:English
Published: Elsevier 2010-06-01
Series:Journal of Lipid Research
Subjects:
myristoylation
palmitoylation
mitochondria
membranes
enzymes
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520410326

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http://www.sciencedirect.com/science/article/pii/S0022227520410326

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