The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.

The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.

Cyan fluorescent proteins (CFP) derived from Aequorea victoria GFP, carrying a tryptophan-based chromophore, are widely used as FRET donors in live cell fluorescence imaging experiments. Recently, several CFP variants with near-ultimate photophysical performances were obtained through a mix of site-...

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Bibliographic Details
Main Authors: Asma Fredj, Hélène Pasquier, Isabelle Demachy, Gabriella Jonasson, Bernard Levy, Valérie Derrien, Yasmina Bousmah, Gallia Manoussaris, Frank Wien, Jacqueline Ridard, Marie Erard, Fabienne Merola
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3487735?pdf=render

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http://europepmc.org/articles/PMC3487735?pdf=render

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