Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses.
BACKGROUND: The true interest of genetic immunisation might have been hastily underestimated based on overall immunogenicity data in humans and lack of parallelism with other, more classical immunisation methods. PRINCIPAL FINDINGS: Using malaria Liver Stage Antigen-3 (LSA-3), we report that genetic...
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doaj-e7faa32e289c45b3ae692c98247555412020-11-25T02:22:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0137e265910.1371/journal.pone.0002659Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses.Pierre DaubersiesBenjamin OllomoJean-Pierre SauzetKarima BrahimiBlanca-Liliana PerlazaWijnand ElingHubert MoukanaPierre RouquetCharles de TaisnePierre DruilheBACKGROUND: The true interest of genetic immunisation might have been hastily underestimated based on overall immunogenicity data in humans and lack of parallelism with other, more classical immunisation methods. PRINCIPAL FINDINGS: Using malaria Liver Stage Antigen-3 (LSA-3), we report that genetic immunization induces in chimpanzees, the closest relative of humans, immune responses which are as scarce as those reported using other DNA vaccines in humans, but which nonetheless confer strong, sterile and reproducible protection. The pattern was consistent in 3/4 immunized apes against two high dose sporozoite challenges performed as late as 98 and 238 days post-immunization and by a heterologous strain. CONCLUSIONS: These results should, in our opinion, lead to a revisiting of the value of this unusual means of immunisation, using as a model a disease, malaria, in which virulent challenges of volunteers are ethically acceptable.http://europepmc.org/articles/PMC2441826?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pierre Daubersies Benjamin Ollomo Jean-Pierre Sauzet Karima Brahimi Blanca-Liliana Perlaza Wijnand Eling Hubert Moukana Pierre Rouquet Charles de Taisne Pierre Druilhe |
spellingShingle |
Pierre Daubersies Benjamin Ollomo Jean-Pierre Sauzet Karima Brahimi Blanca-Liliana Perlaza Wijnand Eling Hubert Moukana Pierre Rouquet Charles de Taisne Pierre Druilhe Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. PLoS ONE |
author_facet |
Pierre Daubersies Benjamin Ollomo Jean-Pierre Sauzet Karima Brahimi Blanca-Liliana Perlaza Wijnand Eling Hubert Moukana Pierre Rouquet Charles de Taisne Pierre Druilhe |
author_sort |
Pierre Daubersies |
title |
Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. |
title_short |
Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. |
title_full |
Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. |
title_fullStr |
Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. |
title_full_unstemmed |
Genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. |
title_sort |
genetic immunisation by liver stage antigen 3 protects chimpanzees against malaria despite low immune responses. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-01-01 |
description |
BACKGROUND: The true interest of genetic immunisation might have been hastily underestimated based on overall immunogenicity data in humans and lack of parallelism with other, more classical immunisation methods. PRINCIPAL FINDINGS: Using malaria Liver Stage Antigen-3 (LSA-3), we report that genetic immunization induces in chimpanzees, the closest relative of humans, immune responses which are as scarce as those reported using other DNA vaccines in humans, but which nonetheless confer strong, sterile and reproducible protection. The pattern was consistent in 3/4 immunized apes against two high dose sporozoite challenges performed as late as 98 and 238 days post-immunization and by a heterologous strain. CONCLUSIONS: These results should, in our opinion, lead to a revisiting of the value of this unusual means of immunisation, using as a model a disease, malaria, in which virulent challenges of volunteers are ethically acceptable. |
url |
http://europepmc.org/articles/PMC2441826?pdf=render |
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