Protocol for intervention-free quantification of protein turnover rate by steady-state modeling

Protocol for intervention-free quantification of protein turnover rate by steady-state modeling

Summary: Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This approach can be used to calculate product...

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Bibliographic Details
Main Authors: Stefania Marcotti, Besaiz Jose Sánchez-Sánchez, Eduardo Serna-Morales, Anca Dragu, María-del-Carmen Díaz-de-la-Loza, Yutaka Matsubayashi, Brian Marc Stramer
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cell biology
Model organisms
Molecular biology
Protein biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000848

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http://www.sciencedirect.com/science/article/pii/S2666166721000848

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