Optimizations of expression and purification of recombinant HIV-1 CRF01_AE p24 protein in Escherichia coli for development of immunodiagnostic assay

Optimizations of expression and purification of recombinant HIV-1 CRF01_AE p24 protein in Escherichia coli for development of immunodiagnostic assay

<p><strong>Background:</strong> Conventional method for confirmation of HIV infection is Western blot. However, it has limitations because of contamination by human cellular antigen and genetic diversity among the HIV-1 subtypes that show indeterminate result and inaccuracy for the...

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Bibliographic Details
Main Authors: Ade P.R. Simaremare, Budiman Bela, Andi Yasmon, Fera Ibrahim
Format: Article
Language:English
Published: Faculty of Medicine Universitas Indonesia 2015-04-01
Series:Medical Journal of Indonesia
Subjects:
expression
HIV-1 CRF01_AE
p24 protein
purification
recombinant protein
Online Access:http://mji.ui.ac.id/journal/index.php/mji/article/view/1166

Internet

http://mji.ui.ac.id/journal/index.php/mji/article/view/1166

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