OPTIMIZATION OF RNA EXTRACTION PROTOCOLS USING DIFFERENT MAIZE TISSUES

OPTIMIZATION OF RNA EXTRACTION PROTOCOLS USING DIFFERENT MAIZE TISSUES

Five RNA extraction methods were tested (CTAB micro extraction, Concert™ - Invitrogen; Concert™ adapted; TRI Reagent® - Sigma; TRI Reagent® adapted) using two different maize tissues (mesocotyl and root), objectifying to establish an efficient method to extract RNA to be later used in gene expressio...

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Bibliographic Details
Main Authors: BRENDA NEVES PORTO, PAULO CÉSAR MAGALHÃES, NÁDIA ALVES CAMPOS, JOSÉ DONIZETI ALVES, MARCELO MURAD MAGALHÃES
Format: Article
Language:English
Published: Associação Brasileira de Milho e Sorgo 2010-08-01
Series:Revista Brasileira de Milho e Sorgo
Subjects:
Nucleic Acids
Zea mays
mesocotyl
root
Online Access:http://rbms.cnpms.embrapa.br/index.php/ojs/article/view/315/366

Internet

http://rbms.cnpms.embrapa.br/index.php/ojs/article/view/315/366

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