Controlling gene activation by enhancers through a drug-inducible topological insulator

Controlling gene activation by enhancers through a drug-inducible topological insulator

While regulation of gene-enhancer interaction is intensively studied, its application remains limited. Here, we reconstituted arrays of CTCF-binding sites and devised a synthetic topological insulator with tetO for chromatin-engineering (STITCH). By coupling STITCH with tetR linked to the KRAB domai...

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Bibliographic Details
Main Authors: Taro Tsujimura, Osamu Takase, Masahiro Yoshikawa, Etsuko Sano, Matsuhiko Hayashi, Kazuto Hoshi, Tsuyoshi Takato, Atsushi Toyoda, Hideyuki Okano, Keiichi Hishikawa
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-05-01
Series:eLife
Subjects:
enhancer
chromatin conformation
gene expression
epigenetic manipulation
CTCF
MYC
Online Access:https://elifesciences.org/articles/47980

Internet

https://elifesciences.org/articles/47980

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