Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of <i>Mycobacterium tuberculosis</i>

Droplet digital PCR (ddPCR) is a third generation of PCR that was recently developed to overcome the challenges of real-time fluorescence-based quantitative PCR (qPCR) in absolute quantification of pathogens. Few studies have been done on tuberculosis (TB) detection and quantification using ddPCR de...

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Bibliographic Details
Main Authors: Raphael Nyaruaba, Jin Xiong, Caroline Mwaliko, Nuo Wang, Belindah J. Kibii, Junping Yu, Hongping Wei
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/8/5/701