Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!

Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!

Abstract Background A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in a dilution series. This method suffers from a seri...

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Bibliographic Details
Main Author: Joel Tellinghuisen
Format: Article
Language:English
Published: BMC 2020-07-01
Series:BMC Bioinformatics
Subjects:
qPCR
Amplification efficiency
Data analysis
Weighted least squares
Statistical errors
Calibration
Online Access:http://link.springer.com/article/10.1186/s12859-020-03604-4

Internet

http://link.springer.com/article/10.1186/s12859-020-03604-4

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