Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!
Abstract Background A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in a dilution series. This method suffers from a seri...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-07-01
|
Series: | BMC Bioinformatics |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12859-020-03604-4 |