Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling
Summary: Succinate functions both as a classical TCA cycle metabolite and an extracellular metabolic stress signal sensed by the mainly Gi-coupled succinate receptor SUCNR1. In the present study, we characterize and compare effects and signaling pathways activated by succinate and both classes of no...
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doaj-e906e89d1b5e4aa098dfeafb3fbc142d2021-06-17T04:46:38ZengElsevierCell Reports2211-12472021-06-013511109246Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signalingMette Trauelsen0Thomas K. Hiron1Da Lin2Jacob E. Petersen3Billy Breton4Anna Sofie Husted5Siv A. Hjorth6Asuka Inoue7Thomas M. Frimurer8Michel Bouvier9Chris A. O’Callaghan10Thue W. Schwartz11Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Maersk Tower, 2200 Copenhagen, DenmarkWellcome Trust Centre for Human Genetics and NIHR Oxford Biomedical Research Centre, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UKWellcome Trust Centre for Human Genetics and NIHR Oxford Biomedical Research Centre, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UKNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Maersk Tower, 2200 Copenhagen, DenmarkDepartment of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, Faculty of Medicine, Université de Montréal, Montréal, QC, CanadaNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Maersk Tower, 2200 Copenhagen, DenmarkNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Maersk Tower, 2200 Copenhagen, DenmarkGraduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, JapanNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Maersk Tower, 2200 Copenhagen, DenmarkDepartment of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, Faculty of Medicine, Université de Montréal, Montréal, QC, CanadaWellcome Trust Centre for Human Genetics and NIHR Oxford Biomedical Research Centre, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UKNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Maersk Tower, 2200 Copenhagen, Denmark; Corresponding authorSummary: Succinate functions both as a classical TCA cycle metabolite and an extracellular metabolic stress signal sensed by the mainly Gi-coupled succinate receptor SUCNR1. In the present study, we characterize and compare effects and signaling pathways activated by succinate and both classes of non-metabolite SUCNR1 agonists. By use of specific receptor and pathway inhibitors, rescue in G-protein-depleted cells and monitoring of receptor G protein activation by BRET, we identify Gq rather than Gi signaling to be responsible for SUCNR1-mediated effects on basic transcriptional regulation. Importantly, in primary human M2 macrophages, in which SUCNR1 is highly expressed, we demonstrate that physiological concentrations of extracellular succinate act through SUCNR1-activated Gq signaling to efficiently regulate transcription of immune function genes in a manner that hyperpolarizes their M2 versus M1 phenotype. Thus, sensing of stress-induced extracellular succinate by SUCNR1 is an important transcriptional regulator in human M2 macrophages through Gq signaling.http://www.sciencedirect.com/science/article/pii/S2211124721006112GPR91SUCNR1succinateM2 macrophagesG proteinGq signaling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mette Trauelsen Thomas K. Hiron Da Lin Jacob E. Petersen Billy Breton Anna Sofie Husted Siv A. Hjorth Asuka Inoue Thomas M. Frimurer Michel Bouvier Chris A. O’Callaghan Thue W. Schwartz |
spellingShingle |
Mette Trauelsen Thomas K. Hiron Da Lin Jacob E. Petersen Billy Breton Anna Sofie Husted Siv A. Hjorth Asuka Inoue Thomas M. Frimurer Michel Bouvier Chris A. O’Callaghan Thue W. Schwartz Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling Cell Reports GPR91 SUCNR1 succinate M2 macrophages G protein Gq signaling |
author_facet |
Mette Trauelsen Thomas K. Hiron Da Lin Jacob E. Petersen Billy Breton Anna Sofie Husted Siv A. Hjorth Asuka Inoue Thomas M. Frimurer Michel Bouvier Chris A. O’Callaghan Thue W. Schwartz |
author_sort |
Mette Trauelsen |
title |
Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling |
title_short |
Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling |
title_full |
Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling |
title_fullStr |
Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling |
title_full_unstemmed |
Extracellular succinate hyperpolarizes M2 macrophages through SUCNR1/GPR91-mediated Gq signaling |
title_sort |
extracellular succinate hyperpolarizes m2 macrophages through sucnr1/gpr91-mediated gq signaling |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2021-06-01 |
description |
Summary: Succinate functions both as a classical TCA cycle metabolite and an extracellular metabolic stress signal sensed by the mainly Gi-coupled succinate receptor SUCNR1. In the present study, we characterize and compare effects and signaling pathways activated by succinate and both classes of non-metabolite SUCNR1 agonists. By use of specific receptor and pathway inhibitors, rescue in G-protein-depleted cells and monitoring of receptor G protein activation by BRET, we identify Gq rather than Gi signaling to be responsible for SUCNR1-mediated effects on basic transcriptional regulation. Importantly, in primary human M2 macrophages, in which SUCNR1 is highly expressed, we demonstrate that physiological concentrations of extracellular succinate act through SUCNR1-activated Gq signaling to efficiently regulate transcription of immune function genes in a manner that hyperpolarizes their M2 versus M1 phenotype. Thus, sensing of stress-induced extracellular succinate by SUCNR1 is an important transcriptional regulator in human M2 macrophages through Gq signaling. |
topic |
GPR91 SUCNR1 succinate M2 macrophages G protein Gq signaling |
url |
http://www.sciencedirect.com/science/article/pii/S2211124721006112 |
work_keys_str_mv |
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