Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation
Aggregation of α-synuclein (αSyn) into proteinaceous deposits is a pathological hallmark of a range of neurodegenerative diseases including Parkinson’s disease (PD). Numerous lines of evidence indicate that the accumulation of toxic oligomeric and prefibrillar αSyn species may underpin the cellular...
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doaj-e91dcc1af7564367b8b5800b2776ae2c2021-04-12T07:09:56ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992021-04-011410.3389/fnmol.2021.659926659926Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and AggregationBlagovesta Popova0Dan Wang1Abirami Rajavel2Karthikeyan Dhamotharan3Diana F. Lázaro4Jennifer Gerke5Joachim F. Uhrig6Michael Hoppert7Tiago F. Outeiro8Tiago F. Outeiro9Tiago F. Outeiro10Tiago F. Outeiro11Gerhard H. Braus12Department of Molecular Microbiology and Genetics, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, GermanyDepartment of Molecular Microbiology and Genetics, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, GermanyDepartment of Molecular Microbiology and Genetics, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, GermanyDepartment of Molecular Microbiology and Genetics, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, GermanyDepartment of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Goettingen, Göttingen, GermanyDepartment of Molecular Microbiology and Genetics, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, GermanyDepartment of Plant Molecular Biology and Physiology, University of Goettingen, Göttingen, GermanyDepartment of General Microbiology, Institute of Microbiology and Genetics, University of Goettingen, Göttingen, GermanyDepartment of Experimental Neurodegeneration, Center for Biostructural Imaging of Neurodegeneration, University Medical Center Goettingen, Göttingen, GermanyMax Planck Institute for Experimental Medicine, Göttingen, GermanyTranslational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne, United KingdomDeutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Göttingen, GermanyDepartment of Molecular Microbiology and Genetics, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, GermanyAggregation of α-synuclein (αSyn) into proteinaceous deposits is a pathological hallmark of a range of neurodegenerative diseases including Parkinson’s disease (PD). Numerous lines of evidence indicate that the accumulation of toxic oligomeric and prefibrillar αSyn species may underpin the cellular toxicity and spread of pathology between cells. Therefore, aggregation of αSyn is considered a priority target for drug development, as aggregation inhibitors are expected to reduce αSyn toxicity and serve as therapeutic agents. Here, we used the budding yeast S. cerevisiae as a platform for the identification of short peptides that inhibit αSyn aggregation and toxicity. A library consisting of approximately one million peptide variants was utilized in two high-throughput screening approaches for isolation of library representatives that reduce αSyn-associated toxicity and aggregation. Seven peptides were isolated that were able to suppress specifically αSyn toxicity and aggregation in living cells. Expression of the peptides in yeast reduced the accumulation of αSyn-induced reactive oxygen species and increased cell viability. Next, the peptides were chemically synthesized and probed for their ability to modulate αSyn aggregation in vitro. Two synthetic peptides, K84s and K102s, of 25 and 19 amino acids, respectively, significantly inhibited αSyn oligomerization and aggregation at sub-stoichiometric molar ratios. Importantly, K84s reduced αSyn aggregation in human cells. These peptides represent promising αSyn aggregation antagonists for the development of future therapeutic interventions.https://www.frontiersin.org/articles/10.3389/fnmol.2021.659926/fullα-synucleinParkinson’s diseaseprotein aggregationoligomerizationpeptide drug discoveryyeast |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Blagovesta Popova Dan Wang Abirami Rajavel Karthikeyan Dhamotharan Diana F. Lázaro Jennifer Gerke Joachim F. Uhrig Michael Hoppert Tiago F. Outeiro Tiago F. Outeiro Tiago F. Outeiro Tiago F. Outeiro Gerhard H. Braus |
spellingShingle |
Blagovesta Popova Dan Wang Abirami Rajavel Karthikeyan Dhamotharan Diana F. Lázaro Jennifer Gerke Joachim F. Uhrig Michael Hoppert Tiago F. Outeiro Tiago F. Outeiro Tiago F. Outeiro Tiago F. Outeiro Gerhard H. Braus Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation Frontiers in Molecular Neuroscience α-synuclein Parkinson’s disease protein aggregation oligomerization peptide drug discovery yeast |
author_facet |
Blagovesta Popova Dan Wang Abirami Rajavel Karthikeyan Dhamotharan Diana F. Lázaro Jennifer Gerke Joachim F. Uhrig Michael Hoppert Tiago F. Outeiro Tiago F. Outeiro Tiago F. Outeiro Tiago F. Outeiro Gerhard H. Braus |
author_sort |
Blagovesta Popova |
title |
Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation |
title_short |
Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation |
title_full |
Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation |
title_fullStr |
Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation |
title_full_unstemmed |
Identification of Two Novel Peptides That Inhibit α-Synuclein Toxicity and Aggregation |
title_sort |
identification of two novel peptides that inhibit α-synuclein toxicity and aggregation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2021-04-01 |
description |
Aggregation of α-synuclein (αSyn) into proteinaceous deposits is a pathological hallmark of a range of neurodegenerative diseases including Parkinson’s disease (PD). Numerous lines of evidence indicate that the accumulation of toxic oligomeric and prefibrillar αSyn species may underpin the cellular toxicity and spread of pathology between cells. Therefore, aggregation of αSyn is considered a priority target for drug development, as aggregation inhibitors are expected to reduce αSyn toxicity and serve as therapeutic agents. Here, we used the budding yeast S. cerevisiae as a platform for the identification of short peptides that inhibit αSyn aggregation and toxicity. A library consisting of approximately one million peptide variants was utilized in two high-throughput screening approaches for isolation of library representatives that reduce αSyn-associated toxicity and aggregation. Seven peptides were isolated that were able to suppress specifically αSyn toxicity and aggregation in living cells. Expression of the peptides in yeast reduced the accumulation of αSyn-induced reactive oxygen species and increased cell viability. Next, the peptides were chemically synthesized and probed for their ability to modulate αSyn aggregation in vitro. Two synthetic peptides, K84s and K102s, of 25 and 19 amino acids, respectively, significantly inhibited αSyn oligomerization and aggregation at sub-stoichiometric molar ratios. Importantly, K84s reduced αSyn aggregation in human cells. These peptides represent promising αSyn aggregation antagonists for the development of future therapeutic interventions. |
topic |
α-synuclein Parkinson’s disease protein aggregation oligomerization peptide drug discovery yeast |
url |
https://www.frontiersin.org/articles/10.3389/fnmol.2021.659926/full |
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