Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set

Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set

Background: The efficiency of isolation and purification of the viral genome is a critical step to the accuracy and reliability of RT-qPCR to detect SARS-CoV-2. However, COVID-19 testing laboratories were overwhelmed by a surge in diagnostic demand that affected supply chains especially in low and m...

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Bibliographic Details
Main Authors: Vinícius Pietta Perez, Wallace Felipe Blohem Pessoa, Bruno Henrique Andrade Galvão, Eduardo Sergio Soares Sousa, Naiara Naiana Dejani, Eloiza Helena Campana, Marilia Gabriela dos Santos Cavalcanti, Vlademir Vicente Cantarelli
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Journal of Clinical Virology Plus
Subjects:
RNA extraction
Direct RT-qPCR
Viral diagnostic
COVID-19
Online Access:http://www.sciencedirect.com/science/article/pii/S2667038021000247

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http://www.sciencedirect.com/science/article/pii/S2667038021000247

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