Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

CTB-MPR is a fusion protein between the B subunit of cholera toxin (CTB) and the membrane-proximal region of gp41 (MPR), the transmembrane envelope protein of Human immunodeficiency virus 1 (HIV-1), and has previously been shown to induce the production of anti-HIV-1 antibodies with antiviral functi...

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Bibliographic Details
Main Authors: Ho-Hsien Lee, Irene Cherni, HongQi Yu, Raimund Fromme, Jeffrey D. Doran, Ingo Grotjohann, Michele Mittman, Shibom Basu, Arpan Deb, Katerina Dörner, Andrew Aquila, Anton Barty, Sébastien Boutet, Henry N. Chapman, R. Bruce Doak, Mark S. Hunter, Daniel James, Richard A. Kirian, Christopher Kupitz, Robert M. Lawrence, Haiguang Liu, Karol Nass, Ilme Schlichting, Kevin E. Schmidt, M. Marvin Seibert, Robert L. Shoeman, John C. H. Spence, Francesco Stellato, Uwe Weierstall, Garth J. Williams, Chunhong Yoon, Dingjie Wang, Nadia A. Zatsepin, Brenda G. Hogue, Nobuyuki Matoba, Petra Fromme, Tsafrir S. Mor
Format: Article
Language:English
Published: International Union of Crystallography 2014-09-01
Series:IUCrJ
Subjects:
X-ray crystallography
femtosecond nanocrystallography
HIV-1
gp41
membrane-proximal region
cholera toxin B subunit
crystallization
free-electron lasers
Online Access:http://scripts.iucr.org/cgi-bin/paper?S2052252514014900

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http://scripts.iucr.org/cgi-bin/paper?S2052252514014900

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