Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis

Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis

Abstract Objective Targeted sequencing of 16S rDNA amplicons is routinely used for microbial community profiling but this method suffers several limitations such as bias affinity of universal primers and short read size. Gene capture by hybridization represents a promising alternative. Here we used...

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Bibliographic Details
Main Authors: Marie Cariou, Céline Ribière, Stéphanie Morlière, Jean-Pierre Gauthier, Jean-Christophe Simon, Pierre Peyret, Sylvain Charlat
Format: Article
Language:English
Published: BMC 2018-07-01
Series:BMC Research Notes
Subjects:
Hybridization capture
Amplicon sequencing
16S
Pea aphid
Microbiota
Online Access:http://link.springer.com/article/10.1186/s13104-018-3559-3

Internet

http://link.springer.com/article/10.1186/s13104-018-3559-3

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