Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA
Generation of recombinant negative-stranded RNA viruses (NSVs) from plasmids involves in vivo reconstitution of biologically active nucleocapsids and faces a unique antisense problem where the negative-sense viral genomic RNAs can hybridize to viral messenger RNAs. To overcome this problem, a positi...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-12-01
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Series: | Viruses |
Subjects: | |
Online Access: | https://www.mdpi.com/1999-4915/12/12/1459 |