Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies

Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR...

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Bibliographic Details
Main Authors: Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Walter Soares Leal, Constância Flávia Junqueira Ayres
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2017-05-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597&lng=en&tlng=en