Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation.

Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation.

Propidium monoazide (PMA)-pretreatment has increasingly been applied to remove the bias from dead or damaged cell artefacts, which could impact the microbiota analysis by high-throughput sequencing. Our study aimed to determine whether a PMA-pretreatment coupled with high-throughput sequencing analy...

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Bibliographic Details
Main Authors: Linh Do Ngoc Nguyen, Pieter Deschaght, Sophie Merlin, Alexandre Loywick, Christophe Audebert, Sabine Van Daele, Eric Viscogliosi, Mario Vaneechoutte, Laurence Delhaes
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5193350?pdf=render

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http://europepmc.org/articles/PMC5193350?pdf=render

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