Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

Abstract Background Cytidine base editors (CBEs), composed of a cytidine deaminase fused to Cas9 nickase (nCas9), enable efficient C-to-T conversion in various organisms. However, current base editors can induce unwanted bystander C-to-T conversions when multiple Cs are present in the ~ 5-nucleotide...

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Bibliographic Details
Main Authors: Zhiquan Liu, Siyu Chen, Huanhuan Shan, Yingqi Jia, Mao Chen, Yuning Song, Liangxue Lai, Zhanjun Li
Format: Article
Language:English
Published: BMC 2020-08-01
Series:BMC Biology
Subjects:
CRISPR/Cas9
Base editor
eA3G
Precision
Online Access:http://link.springer.com/article/10.1186/s12915-020-00849-6

Internet

http://link.springer.com/article/10.1186/s12915-020-00849-6

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