An improved primer set and amplification protocol with increased specificity and sensitivity targeting the Symbiodinium ITS2 region

An improved primer set and amplification protocol with increased specificity and sensitivity targeting the Symbiodinium ITS2 region

The Internal Transcribed Spacer 2 (ITS2) rRNA gene is a commonly targeted genetic marker to assess diversity of Symbiodinium, a dinoflagellate genus of algal endosymbionts that is pervasively associated with marine invertebrates, and notably reef-building corals. Here we tested three commonly used I...

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Bibliographic Details
Main Authors: Benjamin C.C. Hume, Maren Ziegler, Julie Poulain, Xavier Pochon, Sarah Romac, Emilie Boissin, Colomban de Vargas, Serge Planes, Patrick Wincker, Christian R. Voolstra
Format: Article
Language:English
Published: PeerJ Inc. 2018-05-01
Series:PeerJ
Subjects:
Coral Reef
Symbiodinium
Metagenomics
Metabarcoding
Microbial Biology
Next-generation sequencing
Online Access:https://peerj.com/articles/4816.pdf

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