Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

<p>Abstract</p> <p>Background</p> <p>Probe based detection assays form the mainstay of transcript quantification. Problems with these assays include varying hybridization efficiencies of the probes used for transcript quantification and the expense involved. We examined...

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Bibliographic Details
Main Authors: Willey James, Cai Jie, Laird Peter W, Groshen Susan, Beil Stephen J, George Ben, Pagliarulo Vincenzo, Cote Richard J, Datar Ram H
Format: Article
Language:English
Published: BMC 2004-01-01
Series:Molecular Cancer
Online Access:http://www.molecular-cancer.com/content/3/1/5