Flow cytometry-based ex vivo murine NK cell cytotoxicity assay

Summary: Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chro...

Full description

Bibliographic Details
Main Authors: Pamela Wong, Julia A. Wagner, Melissa M. Berrien-Elliott, Timothy Schappe, Todd A. Fehniger
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302495