Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus

Abstract Background In the present work we described the recombinant production and characterization of heterodimeric construction ZnT8-Arg-Trp325 fused to thioredoxin using a high-performance expression system such as Escherichia coli. In addition, we apply this novel recombinant antigen in a non-r...

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Bibliographic Details
Main Authors: Natalia I. Faccinetti, Luciano L. Guerra, Adriana V. Sabljic, Silvina S. Bombicino, Bruno D. Rovitto, Ruben F. Iacono, Edgardo Poskus, Aldana Trabucchi, Silvina N. Valdez
Format: Article
Language:English
Published: BMC 2017-11-01
Series:Microbial Cell Factories
Subjects:
Diabetes mellitus
ZnT8
Autoantibody
Recombinant protein expression
Immunoassays
Escherichia coli
Online Access:http://link.springer.com/article/10.1186/s12934-017-0816-4

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http://link.springer.com/article/10.1186/s12934-017-0816-4

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