Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat
Wheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection...
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2021-04-01
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doaj-ea6c33ef931d40e3b42d5f3f4d83423b2021-04-21T06:10:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.657796657796Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in WheatEva C. Henningsen0Vahid Omidvar1Rafael Della Coletta2Jean-Michel Michno3Erin Gilbert4Feng Li5Marisa E. Miller6Chad L. Myers7Chad L. Myers8Sean P. Gordon9John P. Vogel10John P. Vogel11Brian J. Steffenson12Shahryar F. Kianian13Shahryar F. Kianian14Cory D. Hirsch15Melania Figueroa16Department of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesDepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, United StatesBioinformatics and Computational Biology Graduate Program, University of Minnesota, Minneapolis, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesBioinformatics and Computational Biology Graduate Program, University of Minnesota, Minneapolis, MN, United StatesDepartment of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, United StatesJoint Genome Institute, Walnut Creek, CA, United StatesJoint Genome Institute, Walnut Creek, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesUSDA-ARS Cereal Disease Laboratory, St. Paul, MN, United StatesDepartment of Plant Pathology, University of Minnesota, St. Paul, MN, United StatesCommonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, ACT, AustraliaWheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon. We targeted our analysis to genes with differential expression in T. aestivum and genes suppressed or not affected in B. distachyon and report several processes potentially linked to susceptibility to Pgt, such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to Pgt through removal or modification of putative susceptibility genes.https://www.frontiersin.org/articles/10.3389/fpls.2021.657796/fullsusceptibilityrust (disease)wheattranscriptionco-expressionnon-host |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eva C. Henningsen Vahid Omidvar Rafael Della Coletta Jean-Michel Michno Erin Gilbert Feng Li Marisa E. Miller Chad L. Myers Chad L. Myers Sean P. Gordon John P. Vogel John P. Vogel Brian J. Steffenson Shahryar F. Kianian Shahryar F. Kianian Cory D. Hirsch Melania Figueroa |
spellingShingle |
Eva C. Henningsen Vahid Omidvar Rafael Della Coletta Jean-Michel Michno Erin Gilbert Feng Li Marisa E. Miller Chad L. Myers Chad L. Myers Sean P. Gordon John P. Vogel John P. Vogel Brian J. Steffenson Shahryar F. Kianian Shahryar F. Kianian Cory D. Hirsch Melania Figueroa Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat Frontiers in Plant Science susceptibility rust (disease) wheat transcription co-expression non-host |
author_facet |
Eva C. Henningsen Vahid Omidvar Rafael Della Coletta Jean-Michel Michno Erin Gilbert Feng Li Marisa E. Miller Chad L. Myers Chad L. Myers Sean P. Gordon John P. Vogel John P. Vogel Brian J. Steffenson Shahryar F. Kianian Shahryar F. Kianian Cory D. Hirsch Melania Figueroa |
author_sort |
Eva C. Henningsen |
title |
Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat |
title_short |
Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat |
title_full |
Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat |
title_fullStr |
Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat |
title_full_unstemmed |
Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat |
title_sort |
identification of candidate susceptibility genes to puccinia graminis f. sp. tritici in wheat |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-04-01 |
description |
Wheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon. We targeted our analysis to genes with differential expression in T. aestivum and genes suppressed or not affected in B. distachyon and report several processes potentially linked to susceptibility to Pgt, such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to Pgt through removal or modification of putative susceptibility genes. |
topic |
susceptibility rust (disease) wheat transcription co-expression non-host |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.657796/full |
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