Evaluation of Methods to Assess in vivo Activity of Engineered Genome-Editing Nucleases in Protoplasts

Evaluation of Methods to Assess in vivo Activity of Engineered Genome-Editing Nucleases in Protoplasts

Genome-editing is being implemented in increasing number of plant species using engineered sequence specific nucleases (SSNs) such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated systems (CRISPR/Cas9), Transcription activator like effector nucleases (TALENs), and more...

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Bibliographic Details
Main Authors: Satya Swathi Nadakuduti, Colby G. Starker, Dae Kwan Ko, Thilani B. Jayakody, C. Robin Buell, Daniel F. Voytas, David S. Douches
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Plant Science
Subjects:
genome-editing
CRISPR/Cas9
TALENs
protoplasts
double-stranded oligodeoxynucleotides
NHEJ
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2019.00110/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2019.00110/full

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